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A spectrophotometric procedure for the determination of activity of restriction endonucleases

โœ Scribed by Arvydas A. Janulaitis; Donatas P. Vaitkevitchius

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
945 KB
Volume
116
Category
Article
ISSN
0003-2697

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Continuous spectrophotometric assay for
Continuous spectrophotometric assay for restriction endonucleases using synthetic oligodeoxynucleotides and based on the hyperchromic effect
โœ Timothy R. Waters; Bernard A. Connolly ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 595 KB

A continuous spectrophotometric assay for the EcoRV restriction endonuclease has been developed. The synthetic self-complementary oligonucleotide d(GACGATATCGTC) (which is double stranded under the assay conditions) is used as the substrate. The EcoRV endonuclease recognizes d(GATATC) sequences cutt