Studies on the bipolar argECBH operon of E. coli: Characterization of restriction endonuclease fragments obtained from λdargECBH transducing phages and a ColE1 argECBH plasmid
✍ Scribed by Crabeel, M. ;Charlier, D. ;Glansdorff, N. ;Palchaudhuri, S. ;Maas, W. K.
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 682 KB
- Volume
- 151
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
✦ Synopsis
The isolation of a new type of gamma transducing phage carrying the bipolar argECBH operon of E. coli K12 is described. The argECBH segment is inserted in the phage in a direction which is opposite from that of previously isolated argECBH-carrying phages. A colE1 argECBH plasmid has been constructed. DNA fragments resulting from digestion of these genetic elements with Eco RI and Hind III restriction enzymes have been characterized by agarose gel electrophoresis and electron microscopy, including hetero-duplex analysis. Two fragments are of special significance for studies on the control of arginine synthesis, one of length 9.8 kilobases carrying the whole argECBH region, the other of length 2 kilobases carrying most or all of the control region between argE and argC.