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Determination of Protein–Ligand Binding Constants of a Cooperatively Regulated Tetrameric Enzyme Using Electrospray Mass Spectrometry

✍ Scribed by Cubrilovic, Dragana; Haap, Wolfgang; Barylyuk, Konstantin; Ruf, Armin; Badertscher, Martin; Gubler, Marcel; Tetaz, Tim; Joseph, Catherine; Benz, Jörg; Zenobi, Renato


Book ID
121320747
Publisher
American Chemical Society
Year
2013
Tongue
English
Weight
810 KB
Volume
9
Category
Article
ISSN
1554-8929

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## Abstract We describe an approach for the determination of binding constants for protein–ligand complexes with electrospray ionization mass spectrometry, based on the observation of unbound ligands competing for binding to a protein target. For the first time, dissociation constants lower than pi