Determination of Ligand−Protein Dissociation Constants by Electrospray Mass Spectrometry-Based Diffusion Measurements
✍ Scribed by Clark, Sonya M.; Konermann, Lars
- Book ID
- 126928286
- Publisher
- American Chemical Society
- Year
- 2004
- Tongue
- English
- Weight
- 173 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0003-2700
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## Abstract We describe an approach for the determination of binding constants for protein–ligand complexes with electrospray ionization mass spectrometry, based on the observation of unbound ligands competing for binding to a protein target. For the first time, dissociation constants lower than pi
Electrospray ionization mass spectrometry (ESI-MS) has been used to determine the dissociation constants (K(D)s) and binding stoichiometry for tobramycin and paromomycin with a 27-nucleotide RNA construct representing the A-site of the 16S ribosomal RNA. K(D) values determined by holding the ligand