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Determination of iron in heme compounds: III. Extension and modification of the method

✍ Scribed by Bruce F. Cameron

Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
174 KB
Volume
35
Category
Article
ISSN
0003-2697

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πŸ“œ SIMILAR VOLUMES

Determination of iron in heme compounds:
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The preceding paper in this series (1) describes a rapid and simple determination of total iron in iron porphyrins. The present work extends this method to apply to the naturally occurring hemoproteins, hemoglobin and myoglobin. This requires a digestion to destroy the protein, and some modification

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## Abstract Prompt mixing of acetate buffered Iron(III) and ascorbic acid solutions results in the formation of a short‐lived colored [Fe~3~Ac~6~O] Asc complex showing a maximum absorbance at 550 nm. Mathematical analysis of the kinetics curves allows the elaboration of analytical procedures for th

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A modification of the method of hydroxyproline determination in proteins was devised. The modification consists of the hydrolysis of proteins in 72% perchloric acid of 100Β°C for 2 hr instead of 20 hr, autoclaving in 6 N HCl or 2 N Ba(OH)\*. Determination of hydroxyproline by the modified method does