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Determination of in-vivo cytoplasmic orthophosphate concentration in yeast

✍ Scribed by Uwe Theobald; Jochen Mohns; Manfred Rizzi

Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
410 KB
Volume
10
Category
Article
ISSN
0951-208X

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✦ Synopsis

To investigate in-vivo concentrations of cytoplasmic phosphate, especially during dynamic conditions, a method has been developed that enables reproducible determination of cytopiasmic phosphate from 5 pM up to 30 FM. The method involves fast sampling. spontaneous inactivation of cell metabolism and differential extraction procedure to gain porosity of the outer cell membrane exclusively. To determine very tow cytoplasmic phosphate concentrations, an enzymatic assay was linked to a sensitive spectrophotometric cycling method to increase the detection limit.

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