๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Dynamics of orthophosphate in yeast cytoplasm

โœ Scribed by Uwe Theobald; Jochen Mohns; Manfred Rizzi

Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
403 KB
Volume
18
Category
Article
ISSN
0141-5492

No coin nor oath required. For personal study only.

โœฆ Synopsis

Orthophosphate concentration of baker's yeast Saccharomyces cerevisiae was investigated during dynamic conditions. As an example for those dynamics in cell metabolism the transition from glucose limitation to glucose excess (Crabtree-effect) was choosen. As a result of the metabolic switch from complete to partial oxidative metabolism, the cytoplasmic phosphate concentration increased suddenly from 8.4 mM to a maximum of 17.5 mM and transiently decreased to a minimum of 7.0 mM.

๐Ÿ“œ SIMILAR VOLUMES

Determination of in-vivo cytoplasmic ort
Determination of in-vivo cytoplasmic orthophosphate concentration in yeast
โœ Uwe Theobald; Jochen Mohns; Manfred Rizzi ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 410 KB

To investigate in-vivo concentrations of cytoplasmic phosphate, especially during dynamic conditions, a method has been developed that enables reproducible determination of cytopiasmic phosphate from 5 pM up to 30 FM. The method involves fast sampling. spontaneous inactivation of cell metabolism and

Participation of vacuoles in regulation
Participation of vacuoles in regulation of levels of K+, Mg2+and orthophosphate ions in cytoplasm of the yeastSaccharomyces carlsbergensis
โœ Lidia P. Lichko; Lev A. Okorokov; Igor S. Kulaev ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Springer ๐ŸŒ English โš– 453 KB

Intracellular distributions of K +, Mg 2+ and orthophosphate under various conditions of cultivation or incubation of the yeast Saccharomyces carlsbergensis were studied by differential extraction of ion pools. The decisive role of vacuolar compartmentation of ions in regulation of K +, Mg 2+ and or

Nucleo-cytoplasmic interactions in the p
Nucleo-cytoplasmic interactions in the petite negative yeast Schizosaccharomyces pombe
โœ Del Giudice, L. ;Wolf, K. ;Buono, C. ;Manna, F. ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 450 KB

In the petite positive yeast, Saccharomyces cerevisiae, cycloheximide selectively inhibits protein synthesis on cytoplasmic ribosomes, and, as a consequence, nuclear DNA synthesis. Mitochondrial DNA, however, is synthesized for d~6 h after cessation of protein synthesis. In this paper we show that i