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A modified colorimetric method for the determination of orthophosphate in the presence of high ATP concentrations

✍ Scribed by Pilar González-Romo; Sobeida Sánchez-Nieto; Marina Gavilanes-Ruíz

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
328 KB
Volume
200
Category
Article
ISSN
0003-2697

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✦ Synopsis

We describe a modified colorimetric method that quantitates inorganic phosphate linearly up to 60 nmol, with high stability of the developed color and with a low interference by ATP concentration (up to 30 mM). This method is very suitable for use in ATPase enzymatic assays, especially with enzymes that have low specific activities and (or) high Km values for ATP.

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