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31P NMR Methods for the Direct Determination of ADP in the Presence of ATP

✍ Scribed by Dafna Ben-Bashat; Hadassah Shinar; Gil Navon

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
174 KB
Volume
110
Category
Article
ISSN
1064-1866

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✦ Synopsis

A new method is presented for the direct measurement of the amount of ADP in the presence of ATP by selectively eliminating the alpha- and gamma-ATP signals. The method is compared with other methods, both experimentally and theoretically, using the product-operator formalism. An analysis of the effect of beta 1 inhomogeneity on the efficiency of the methods is also given. Experimental results obtained using adiabatic pulses to compensate for such effects are shown. The accuracy of this new method is demonstrated by measuring various ADP concentrations in a series of solutions containing ADP and ATP.

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