Determination of fructose and fructose-yielding carbohydrates with cold anthrone
β Scribed by Emile Van Handel
- Book ID
- 107710809
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 98 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0003-2697
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A sequential method for determination of glucose and fructose involving the u8e of enzymes (hexokinase and glucose-6-phosphate dehydrogenase) immobilized on controlled-pore glass is proposed. The flow is selected so as to determine glucose or both sugars by fluorimetric determination of the NADH for
Immobdlzed sorbltol dehydrogenase was used as a reactor m a column hqmd chromatograplnc system for the selective detection of sucrose and fructose Sorbltol dehydrogenase was lmmoblhzed on controlled-pore glass The sugars were separated on a cation-exchange resm column with a mobde phase contammg 0 1