An analytical method is presented for the quantitative determination of certain major and modified bases in unfractionated rat liver transfer RNA (tRNA). tRNA was hydrolyzed with perchloric acid, and the liberated bases were separated by high-pressure liquid chromatography. Bases were selectively de
Determination of E. gracilis mRNA base composition using high-pressure liquid chromatography
โ Scribed by Kenneth H. Falchuk; Christian Hardy
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 467 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
The purine and pyrimidine bases of E. gracilis have been separated using a high-pressure liquid chromatograph (HPLC). Each base was unambiguously identified by its characteristic elution profile and UV absorption spectrum. This method allows for the study of the base composition of mRNAs altered by pathological processes, exemplified here by the analysis and comparison of mRNAs from zinc sufficient and deficient organisms.
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