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Determination of E. gracilis mRNA base composition using high-pressure liquid chromatography

โœ Scribed by Kenneth H. Falchuk; Christian Hardy


Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
467 KB
Volume
89
Category
Article
ISSN
0003-2697

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โœฆ Synopsis


The purine and pyrimidine bases of E. gracilis have been separated using a high-pressure liquid chromatograph (HPLC). Each base was unambiguously identified by its characteristic elution profile and UV absorption spectrum. This method allows for the study of the base composition of mRNAs altered by pathological processes, exemplified here by the analysis and comparison of mRNAs from zinc sufficient and deficient organisms.


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