A quantitative assay for the diphenol oxidase activity of tyrosinase (EC 1.14.18.1) using high-pressure liquid chromatography with electrochemical detection is described. The assay is based on the observation (M. Sugumaran, 1986, Biochemistry 25, 4489-4492) that tyrosinase catalyzes the oxidative de
Determination of adenosine deaminase activity using high-pressure liquid chromatography
โ Scribed by Joseph Uberti; James J. Lightbody; Robert M. Johnson
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 432 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
A method of assaying adenosine deaminase, using high-pressure liquid chromatography to isolate products, has been developed, which has several advantages over available procedures. The method has inherently low background values, affording high sensitivity. Ten picomoles of product can be reliably detected, so that as little as 0.008 unit of enzyme can be determined. Up to six samples per hour can be assayed. The procedure has been applied to erythrocytes and lymphocytes.
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