๐”– Bobbio Scriptorium
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Determination of adenosine deaminase activity using high-pressure liquid chromatography

โœ Scribed by Joseph Uberti; James J. Lightbody; Robert M. Johnson


Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
432 KB
Volume
80
Category
Article
ISSN
0003-2697

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โœฆ Synopsis


A method of assaying adenosine deaminase, using high-pressure liquid chromatography to isolate products, has been developed, which has several advantages over available procedures. The method has inherently low background values, affording high sensitivity. Ten picomoles of product can be reliably detected, so that as little as 0.008 unit of enzyme can be determined. Up to six samples per hour can be assayed. The procedure has been applied to erythrocytes and lymphocytes.


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