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Quantitative determination of transfer RNA base composition using high-pressure liquid chromatography

✍ Scribed by John M. Essigmann; William F. Busby Jr.; Gerald N. Wogan

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
665 KB
Volume
81
Category
Article
ISSN
0003-2697

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✦ Synopsis

An analytical method is presented for the quantitative determination of certain major and modified bases in unfractionated rat liver transfer RNA (tRNA). tRNA was hydrolyzed with perchloric acid, and the liberated bases were separated by high-pressure liquid chromatography. Bases were selectively detected in tRNA hydrolysates at wavelengths near their uv-absorption maxima. Recovery values for individual bases generally were in the 80-100% range. The composition of rat liver tRNA with respect to 10 bases was determined. and the levels ofthese bases were in agreement with published values determined by other methods.

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