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Determination of clenbuterol in bovine liver by enzyme immunoassay

✍ Scribed by S.D. Bucknall; A.L. MacKenzie; M.J. Sauer; D.J. Everest; R. Newman; R. Jackman

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
274 KB
Volume
275
Category
Article
ISSN
0003-2670

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✦ Synopsis

Procedures are described for optmusmg and vabdatmg an ELISA method for measurmg clenbuterol residues m bovme bver wlthout pnor sample ennchment Optunal assay conddlons are obtamed by premcubatmg bver supematant wllth an unmoblhsed antibody rmed to clenbuterol-ovalbumen After further mcubatlon wrth a salbutamol-peroxldase coqlugate, &our IS developed usmg a tetramethylbenzldene substrate The assay wll pemut measurement of clenbuterol residues m hver at the maxmmm residue level of 05 ng g-' Hnth a confidence of > 99% I(eywords Enzymatx methods, Immunoassay, &Agomsts, Bovme bver, Clenbuterol, ELISA method

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