Extraction and clean-up of the β-agonist salbutamol from liver and its determination by enzyme immunoassay
✍ Scribed by L. Howells; M. Sauer; R. Sayer; D. Clark
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 375 KB
- Volume
- 275
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
Abstrael A method has been developed for the extractlon and unmunoassay of salbutamol m hver The procedure involves dIgestion of hver urlth Subtdlsm protease at pH 10 5, clar&a~on by acid preapltatlon and centnfugatlon The salbutamol m the supematant was concentrated onto a 2-g Cls "Bond Elut" cartridge at pH 9 1 f 0 3, eluted with 99 5% methanol and the eluate concentrated and quanhtated by enzyme nnmunoassay The method was vahdated usmg tissues m which salbutamol had been incurred and trues sprkcd wtth salbutamol The mean recovery from hver spked at 1 pg kg-' was 57 f 8% (S D ) Statistical analysti of the results for 18 batches of samples enabled the calculation of the probab&y of false negatives (sensltlvlty) and of false posltnres (spec&' ic~ty) of the method The probablhty of false negatives among samples contammg 1 pg kg-' was 1% and that of false positives was 2 5% The method was used successfully on incurred tissues, and IS used routinely m this laboratory for screening samples taken m compliance urlth the requirements of the EC Residues Directive (86/469) Keyworuk Enzymatic methods, Immunoassay, &Agonists, Lver tissue, Salbutamol