Determination of Procymidone in vegetables by a commercial competitive inhibition enzyme immunoassay
✍ Scribed by Amadeo R. Fernandez-Alba; Antonio Valverde; Ana Agüera; Mariano Contreras; Dolores Rodriguez
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 499 KB
- Volume
- 311
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
An ELISA commercial kit was used to quantitate residues of Procymidone at very low levels (< 20 pg/kg) in pepper samples. Samples were extracted with ethyl acetate-sodium sulphate and an aliquot is evaporated to dryness and reconstituted in 10 ml of light petroleum. Sample clean up is accomplished by aspirating 2 ml of the light petroleum extract through a silica gel solid phase disposable cartridge. Following aspiration, the sample was eluted with 2 ml of ethyl ether-petroleum ether (1:l). The eluted fraction was evaporated and dissolved with sonication in 2 ml of water before immunoassay analysis. The limit of Procymidone detection was 0.6 pg/kg. The assay logarithmic response was linear from 5 to 80 pg/kg of Procymidone residue. Irma-assay percentage coefficients of variation (%C.V.) ranged from 3.5 to 18.0 and inter-assays %C.V.s varied from 8.0 to 16.0. Comparison studies between gas chromatography with electron capture detection and ELBA analyses showed an acceptable coincidence at levels of 8 pg/kg. However, lower levels of ca. 2 pg/kg were detectable by ELISA only. No cross-reactivity problems were found in the ELBA test associated with the presence of other dichloroaniline derivatives and other pesticides studied.