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Determination of 5-hydroxymethyl-2-furaldehyde in honey by micellar electrokinetic capillary chromatography

✍ Scribed by Claudio Corradini; Danilo Corradini

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
265 KB
Volume
6
Category
Article
ISSN
1040-7685

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✦ Synopsis

Abstract

Methodology using micellar electrokinetic capillary chromatography (MECC) to determine 5‐hydroxymethyl‐2‐furaldehyde (HMF) as a degradation product of carbohydrates in aged and thermal treated honey is described. Sodium dodecyl sulfate was employed as the anion surfactant in a phosphate buffer, pH 7.5. The sample preparation was limited to dissolution of honey in water and addition of an internal standard solution (2‐furylmethylketone). The method was highly selective and exhibited high reporducibility, accuracy, and short analysis time.

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A method for the determination of 2-furaldehyde (F) and Shydroxymethyl-2-furaldehyde (HMF) in beer by liquid chromatography (LC) is described. The method is based on the formation of the 2,4-dinitrophenylhydrazones of carbonyl compounds and subsequent reversed-phase separation of these derivatives.