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Detection of the JAK2 V617F Mutation by LightCycler PCR and Probe Dissociation Analysis

✍ Scribed by Lay, Marla; Mariappan, Rajan; Gotlib, Jason; Dietz, Lisa; Sebastian, Siby; Schrijver, Iris; Zehnder, James L.


Book ID
125426795
Publisher
American Society for Investigative Pathology
Year
2006
Tongue
English
Weight
558 KB
Volume
8
Category
Article
ISSN
1525-1578

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JAK2V617F is a common mutation in chronic myeloproliferative diseases (CMPDs). We have developed a system utilizing JAK2V617Fspecific guanine quenching probe (QP-system) to detect JAK2V617F. With QP-system, results can be obtained from 100 l of blood within 90 min. We compared QP-system with direct