✦ LIBER ✦

Detection of p53 gene mutations in oral squamous cell carcinomas of a black African population sample

✍ Scribed by Estrelita J. van Rensburg; Susan Engelbrecht; Willem F. P. van Heerden; Maritha J. Kotze; Erich J. Raubenheimer

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 182 KB
Volume: 11
Category: Article
ISSN: 1059-7794
DOI: 10.1002/(sici)1098-1004(1998)11:1<39::aid-humu6>3.0.co;2-g

No coin nor oath required. For personal study only.

✦ Synopsis

Mutations in the p53 gene have been reported in head and neck carcinomas. We determined the p53 mutation profile in 55 oral squamous cell carcinomas (OSCCs) from a black African population sample. DNA from all the patients were investigated using PCR amplification of the p53 gene (exons 5-9), followed by heteroduplex single-stranded conformational polymorphism (HEX-SSCP) analysis on the PCR products. Direct sequencing was performed on cases where mutations were identified. The results showed mutations in 13 of 55 (23.6%) tumours. Eleven of 13 (85%) were single base pair substitutions (9 transitions and 2 transversions), and 2 were deletions. Two novel mutations were identified: a large 63-base pair deletion, and a single base pair substitution. The mutations in our study occurred outside the head and neck tumour hot spot region (codons 238-248).

📜 SIMILAR VOLUMES

p53 gene mutations in sequential oral ep

p53 gene mutations in sequential oral epithelial dysplasias and squamous cell carcinomas

✍ Shahnavaz, S. A.; Regezi, J. A.; Bradley, G.; Dub�, I. D.; Jordan, R. C. K. 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 206 KB 👁 1 views

Previous studies of oral cancer have suggested that alterations of the p53 tumour suppressor gene occur early in the precancerous stage of development. However, these observations have been based on cross-sectional assessment of abnormal p53 protein staining by immunohistochemistry and may not neces

Characteristics of mutations in the p53

Characteristics of mutations in the p53 gene of oral squamous-cell carcinomas associated with betel-quid chewing in Sri Lanka

✍ Itsuo Chiba; Malsantha Muthumala; Yutaka Yamazaki; Asad Uz Zaman; Tadashi Iizuka 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 French ⚖ 73 KB 👁 2 views

Oral squamous-cell carcinoma (SCC) is the most common neoplasm in Sri Lanka, accounting for approximately 30% of all cancers in males. Epidemiologic evidence indicates that there is an unequivocal relationship between betel chewing and oral carcinogenesis, suggesting that there may be specific genet

Mutations of the p53 gene in oral squamo

Mutations of the p53 gene in oral squamous-cell carcinomas from sudanese dippers of nitrosamine-rich toombak and non-snuff-dippers from the Sudan and Scandinavia

✍ Salah O. Ibrahim; Endre N. Vasstrand; Anne C. Johannessen; Ali M. Idris; Bengt M 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 French ⚖ 100 KB 👁 1 views

Using PCR-SSCP/DNA sequencing methods, we analyzed 14 oral squamous-cell carcinomas (OSCCs) and 8 premalignant oral lesions from different Sudanese patients for prevalence of mutations in exons 5 to 9 of the p53 gene in relation to toombak-dipping status. OSCCs (14 from Sudan, 28 from Scandinavia),

Characteristics of mutations in the p53

Characteristics of mutations in the p53 gene of oral squamous-cell carcinomas associated with betel-quid chewing in Sri Lanka. Int. J. Cancer 77, 839–842 (1998)

✍ I. Chiba; M. Muthumala; Y. Yamazaki; A.U. Zaman; T. Iizuka; A. Amemiya; T. Shiba 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 French ⚖ 26 KB 👁 2 views

In Table I of the above-noted article, the column head ''Betel-liquid'' should have been ''betel-quid''. The corrected Table is reprinted here.

Prevalence of HPV infection in esophagea

Prevalence of HPV infection in esophageal squamous cell carcinoma in Chinese patients and its relationship to the p53 gene mutation

✍ De He; De-Kai Zhang; King-Yin Lam; Lily Ma; Hextan Yuen-Sheung Ngan; Stephanie S 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 204 KB 👁 2 views

Human papillomavirus (HPV), in particular types 16 and 18, is positively associated with anogenital cancers and may be an important etiologic factor in their pathogenesis. The goal of our study was to investigate the role of HPV infection in the pathogenesis of esophageal squamous cell carcinoma (ES

p53 mutations in relation to human papil

p53 mutations in relation to human papillomavirus type 16 infection in squamous cell carcinomas of the head and neck

✍ Andrea G.M. Scholes; Triantafillos Liloglou; Peter J.F. Snijders; C. Anthony Har 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 66 KB 👁 1 views