𝔖 Bobbio Scriptorium
✦   LIBER   ✦

p53 gene mutations in sequential oral epithelial dysplasias and squamous cell carcinomas

✍ Scribed by Shahnavaz, S. A.; Regezi, J. A.; Bradley, G.; Dub�, I. D.; Jordan, R. C. K.

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
206 KB
Volume
190
Category
Article
ISSN
0022-3417

No coin nor oath required. For personal study only.

✦ Synopsis

Previous studies of oral cancer have suggested that alterations of the p53 tumour suppressor gene occur early in the precancerous stage of development. However, these observations have been based on cross-sectional assessment of abnormal p53 protein staining by immunohistochemistry and may not necessarily reflect gene changes. The purpose of this longitudinal study was to examine the changes in the p53 gene in progressive, sequential epithelial dysplasias and carcinomas from the oral cavity. The study analysed 24 formalin-fixed, paraffin-embedded tissue biopsies from ten patients with two or more temporally distinct lesions from the same site in the oral cavity with the diagnosis of hyperkeratosis, epithelial dysplasia, carcinoma in situ or squamous cell carcinoma. Exons 5-8 of the p53 gene were amplified from genomic DNA using intronic primers and directly sequenced using fluorescent-labelled primers. Standard immunohistochemistry with the DO7 monoclonal antibody was used to detect mutant and wild-type p53 protein. Mutations of the p53 gene were identified in 9 of 24 samples. Eight were missense mutations and one occurred at a splice site. In six patients, mutations of the p53 gene occurred late after the transformation of epithelial dysplasia to carcinoma. In two patients with progressive dysplasia, but who had yet to develop invasive carcinoma, p53 missense mutations occurred at the carcinoma in situ stage in one case and in a moderate dysplasia in the other. There was an inconsistent relationship between gene mutations and the level of p53 protein staining by immunohistochemistry. It is concluded that during oral carcinogenesis, p53 gene mutations seem to occur relatively late and are associated with transformation to the invasive phenotype.

📜 SIMILAR VOLUMES

p53 gene mutations in oral carcinomas fr
p53 gene mutations in oral carcinomas from India
✍ Arasambattu K. Munirajan; Yuko Tutsumi-Ishii; Bagavathi K. C. Mohanprasad; Yasum 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 French ⚖ 437 KB 👁 2 views

In this study, we analyzed 53 oral squamous-cell carcinomas among Indians for the presence of alterations in the tumorsuppressor gene p53 by PCR-SSCP and sequencing methods. Our results showed that 21% (I 1/53) of oral carcinomas analyzed carried mutations within the exons 5-8 of the p53 gene. We h

Detection of p53 gene mutations in oral
Detection of p53 gene mutations in oral squamous cell carcinomas of a black African population sample
✍ Estrelita J. van Rensburg; Susan Engelbrecht; Willem F. P. van Heerden; Maritha 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 182 KB 👁 2 views

Mutations in the p53 gene have been reported in head and neck carcinomas. We determined the p53 mutation profile in 55 oral squamous cell carcinomas (OSCCs) from a black African population sample. DNA from all the patients were investigated using PCR amplification of the p53 gene (exons 5-9), follow

Circulating p53 antibodies, p53 gene mut
Circulating p53 antibodies, p53 gene mutational profile and product accumulation in esophageal squamous-cell carcinoma in India
✍ Ranju Ralhan; Sonia Arora; Tushar Kant Chattopadhyay; Nootan Kumar Shukla; Meera 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 66 KB 👁 1 views

## Esophageal cancer (EC) in the Indian population presents in advanced stages with poor prognosis and warrants the identification of a non-invasive marker for early detection and better prognostic assessment. We have previously reported high prevalence of p53 protein accumulation in esophageal squ

Characteristics of mutations in the p53
Characteristics of mutations in the p53 gene of oral squamous-cell carcinomas associated with betel-quid chewing in Sri Lanka
✍ Itsuo Chiba; Malsantha Muthumala; Yutaka Yamazaki; Asad Uz Zaman; Tadashi Iizuka 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 French ⚖ 73 KB 👁 2 views

Oral squamous-cell carcinoma (SCC) is the most common neoplasm in Sri Lanka, accounting for approximately 30% of all cancers in males. Epidemiologic evidence indicates that there is an unequivocal relationship between betel chewing and oral carcinogenesis, suggesting that there may be specific genet