Detection of Low Molecular Weight IReduced Zein Polypeptides Separated by Polyacrylamide Gel Electrophoresis
β Scribed by SHELA GORINSTEIN; GEORGE V. QUICKE; ANNE M. PHILLIPS
- Book ID
- 108811595
- Publisher
- Institute of Food Technologists
- Year
- 1986
- Tongue
- English
- Weight
- 249 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0022-1147
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π SIMILAR VOLUMES
A convenient method was established for the M(r) estimation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of large polypeptides in the range from 250,000 to 600,000. Such polypeptides had previously been shown to migrate improperly in various gel electrophoresis systems. In a conventi
Gel filtration, the chromatographic separation of proteins on columns of gel particles of various nature, has empirically been found to constitute a useful tool for molecular size determination (l-6), as indicated by a variety of applications. Few attempts have been made to utilize the interaction o