Molecular weight estimation of human erythropoietin by SDS-polyacrylamide gel electrophoresis
✍ Scribed by Manuel Dorado; Joaquín Espada; Arturo A. Langton; Nora C. Brandan
- Book ID
- 115766253
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 371 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0006-2944
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📜 SIMILAR VOLUMES
Gel filtration, the chromatographic separation of proteins on columns of gel particles of various nature, has empirically been found to constitute a useful tool for molecular size determination (l-6), as indicated by a variety of applications. Few attempts have been made to utilize the interaction o
As shown previously (1)) electrophoresis in polyacrylamide gels in the presence of the anionic detergent sodium dodecyl sulfate (SDS) is a useful tool for the determination of molecular weights of proteins and polypeptide chains. The method is particularly suited to the calculation of molecular weig
The utilization of 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) as a fluorescent label for proteins in SDS polyacrylamide gel electrophresis is described. The procedure is very sensitive and detects disks containing as little as 1 ng of protein. The fluorescence signal is linear with concentration o