The utilization of 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) as a fluorescent label for proteins in SDS polyacrylamide gel electrophresis is described. The procedure is very sensitive and detects disks containing as little as 1 ng of protein. The fluorescence signal is linear with concentration o
Estimation of the molecular weights of acidic mucopolysaccharides by polyacrylamide gel electrophoresis
โ Scribed by J.C. Hilborn; P.A. Anastassiadis
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 278 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A nonlinear relationship between polyacrylamide gel electrophoresis (PAGE) retardation coefficients (KR) and molecular weights has been observed during analysis of several multimeric proteins. Although the deviation from linearity over a wide range of molecular weights is slight, it can lead to sign
A convenient method was established for the M(r) estimation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of large polypeptides in the range from 250,000 to 600,000. Such polypeptides had previously been shown to migrate improperly in various gel electrophoresis systems. In a conventi