Molecular weight estimation of polypeptides by SDS-polyacrylamide gel electrophoresis: Further data concerning resolving power and general considerations
✍ Scribed by Arnold L. Shapiro; Jacob V. Maizel Jr.
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 585 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
As shown previously (1)) electrophoresis in polyacrylamide gels in the presence of the anionic detergent sodium dodecyl sulfate (SDS) is a useful tool for the determination of molecular weights of proteins and polypeptide chains. The method is particularly suited to the calculation of molecular weights of each component of a mixture of polypeptides or proteins of similar molecular weights and to newly synthesized polypeptides. Molecular weight differences as small as 5% are readily detected by use of a gel fractionator
(2) and double-labeling. Tritiated or Cl"amino acids, iodination with Ilz5, carboxymethylation with C"-iodoacetamide, or acetylation with C?-acetic anhydride have been used to produce labeled polypeptides for analysis by this system.
It is the purpose of this communication to present the data and results of experiments designed to describe further the way in which this electrophoretic system operates. MATERIALS AND METHODS cr-Chymotrypsinogen was purchased from Boehringer and trypsin from Worthington Biochemical Corporation. C4-Labeled y-globulin was obtained from the secreted material of MPC-11 mouse plasma cell tumors (3). Tritiated, soluble, lens proteins were produced by labeling whole rabbit lenses with tritiated amino acids in organ culture. ' Recipient USPHS Career Development Award.