Separation of low molecular weight RNA by polyacrylamide gel electrophoresis
β Scribed by Frederick Varricchio; Henry J. Ernst
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 728 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
Gel filtration, the chromatographic separation of proteins on columns of gel particles of various nature, has empirically been found to constitute a useful tool for molecular size determination (l-6), as indicated by a variety of applications. Few attempts have been made to utilize the interaction o
## Abstract Lowβmolecularβweight (LMW) RNA profiles, which include ribosomal and transfer RNA molecules with similar small sizes, are molecular signatures of microorganisms with a great potential in microbial identification. The greatest resolution of these profiles was achieved by staircase electr
A nonlinear relationship between polyacrylamide gel electrophoresis (PAGE) retardation coefficients (KR) and molecular weights has been observed during analysis of several multimeric proteins. Although the deviation from linearity over a wide range of molecular weights is slight, it can lead to sign