A convenient method was established for the M(r) estimation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of large polypeptides in the range from 250,000 to 600,000. Such polypeptides had previously been shown to migrate improperly in various gel electrophoresis systems. In a conventi
Application of sodium dodecyl sulfate-gel electrophoresis to low molecular weight polypeptides
β Scribed by Jianmin Huang; Harry R. Matthews
- Book ID
- 107714901
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 693 KB
- Volume
- 188
- Category
- Article
- ISSN
- 0003-2697
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We have described modifications of apparatus and technique of preparative sodium dodecyl sulfate-gel electrophoresis which improve the resolution of very large proteins. These modifications permit the resolution of several components of apolipoprotein B from human blood plasma.
A sodium dodecyl sulfate-polyacrylamide gradient gel system is described. It combines a linear gradient in polyacrylamide from 10.2 to 30.2% in the separating gel and the discontinuous ammediol/glycine buffer system suggested by Bury (A. F. Bury, 1981, .I. Chromatogr. 213, 49 I-500). This urea-free
Polyacrylamide-gel electrophoresis of low molecular weight DNS-peptides was performed in the presence of 0.1% sodium dodecyl sulfate and 8 M urea. This procedure enabled an estimation of the molecular weight of peptides at the nanomole level without staining. A linear relationship between molecular