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Application of the dansylation reaction to the characterization of low molecular weight peptides by dodecyl sulfate-polyacrylamide-gel electrophoresis

✍ Scribed by Taiji Kato; Makoto Sasaki; Shigeru Kimura

Publisher: Elsevier Science
Year: 1975
Tongue: English
Weight: 508 KB
Volume: 66
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90618-1

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✦ Synopsis

Polyacrylamide-gel electrophoresis of low molecular weight DNS-peptides was performed in the presence of 0.1% sodium dodecyl sulfate and 8 M urea. This procedure enabled an estimation of the molecular weight of peptides at the nanomole level without staining. A linear relationship between molecular weight and mobility was obtained over a molecular weight range of 1,000-l 2.000, although a few anomalous peptides (e.g., glucagon and insulin) and small peptides of molecular weight less than 1,000 deviated from linearity. The N-terminal amino acid of a peptide can be determined in combination with thin layer chromatography on polyamide sheets. The usefulness of this procedure for checking small amounts of contaminant in an oligopeptide sample was also noted.

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