Reliability of molecular weight determination of proteins by polyacrylamide gradient gel electrophoresis in the presence of sodium dodecyl sulfate
โ Scribed by Patrick Lambin
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 661 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A method for resolving an overlapped polypeptide pattern of sodium dodecyl sulfate-polyacrylamide gel electrophoresis was described. The procedure was essentially a Gaussian fitting using the least squares method, and could resolve more than 20 overlapped components simultaneously. The applicability
A convenient method was established for the M(r) estimation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of large polypeptides in the range from 250,000 to 600,000. Such polypeptides had previously been shown to migrate improperly in various gel electrophoresis systems. In a conventi