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Improved separation of high-molecular-weight proteins by preparative sodium dodecyl sulfate-gel electrophoresis: Application to apolipoprotein B

✍ Scribed by David A. Hardman; John P. Kane


Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
530 KB
Volume
105
Category
Article
ISSN
0003-2697

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✦ Synopsis


We have described modifications of apparatus and technique of preparative sodium dodecyl sulfate-gel electrophoresis which improve the resolution of very large proteins. These modifications permit the resolution of several components of apolipoprotein B from human blood plasma.


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Preparative elution of proteins from nit
✍ B.S. Parekh; H.B. Mehta; M.D. West; R.C. Montelaro πŸ“‚ Article πŸ“… 1985 πŸ› Elsevier Science 🌐 English βš– 769 KB

Various conditions were analyzed and optimized for the preparative elution of proteins from nitrocellulose membranes after transfer from sodium dodecyl sulfate (SDS)-polyacrylamide gels. The efficiency of elution was best using pyridine or acetonitrile elution solvents, intermediate for buffer conta