Various conditions were analyzed and optimized for the preparative elution of proteins from nitrocellulose membranes after transfer from sodium dodecyl sulfate (SDS)-polyacrylamide gels. The efficiency of elution was best using pyridine or acetonitrile elution solvents, intermediate for buffer conta
β¦ LIBER β¦
Improved separation of high-molecular-weight proteins by preparative sodium dodecyl sulfate-gel electrophoresis: Application to apolipoprotein B
β Scribed by David A. Hardman; John P. Kane
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 530 KB
- Volume
- 105
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
We have described modifications of apparatus and technique of preparative sodium dodecyl sulfate-gel electrophoresis which improve the resolution of very large proteins. These modifications permit the resolution of several components of apolipoprotein B from human blood plasma.
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