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The effects of high salt concentrations in the samples on molecular weight determination in sodium dodecyl sulfate polyacrylamide gel electrophoresis

✍ Scribed by Yew Phew See; Peter M. Olley; Dr. George Jackowski


Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
966 KB
Volume
6
Category
Article
ISSN
0173-0835

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A method is described for obtaining a sample-stacking effect before sodium dodecyl sulfate-agarose gel electrophoresis. The method requires no novel equipment and uses a discontinuous polyacrylamide-agarose horizontal slab gel system. With the buffers selected, a complex mixture of proteins can be s