Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients

✍ Scribed by Eren, E.; Aytac, U.; Tetik, E.; Akman, O.; Kansu, E.; Gunduz, U.

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 181 KB
• Volume: 63
• Category: Article
• ISSN: 0361-8609
• DOI: 10.1002/(sici)1096-8652(200002)63:2<85::aid-ajh5>3.0.co;2-l

✦ Synopsis

Cytogenetic and molecular studies were performed on 20 interferon-␣ receiving Turkish chronic myelocytic leukemia patients. Four different restriction endonucleases and bcr-G probe were used for southern blot analysis to detect rearrangements of the bcr gene. The RT-PCR method was also applied to detect chimeric bcr/abl mRNA. Seventeen patients showed a chromosomal break within the 5.8 kb M-bcr region by southern blot analysis while three cases out of 20 have not shown any rearrangement. These three cases were further analysed by RT-PCR and they were also found to be carrying the Philadelphia translocation (Ph). However, in four years of follow-up this RT-PCR positivity has disappeared, which suggests an elimination of Ph clone with prolonged interferon-␣ treatment.