Detection of bacterial and mycoplasma contamination in cell cultures by polymerase chain reaction
✍ Scribed by Marijke Spaepen; Alexander Fidèle Angulo; Peter Marynen; Jean-Jacques Cassiman
- Book ID
- 109317883
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 368 KB
- Volume
- 99
- Category
- Article
- ISSN
- 0378-1097
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The in situ staining method of Chen (1977) for the detection of mycoplasma contaminants in tissue cultures was tested in cultures of human skin fibroblasts after controlled contamination with Mycoplasma arginini. It is concluded that this method is reliable only at infection rates of 100% or higher,