𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Detection of mycoplasma contamination in tissue cultures by fluorescence microscopy

✍ Scribed by Maya Thangavelu; H. Ernø; A. J. Therkelsen

Publisher
Springer
Year
1979
Tongue
English
Weight
184 KB
Volume
47
Category
Article
ISSN
0340-6717

No coin nor oath required. For personal study only.

✦ Synopsis

The in situ staining method of Chen (1977) for the detection of mycoplasma contaminants in tissue cultures was tested in cultures of human skin fibroblasts after controlled contamination with Mycoplasma arginini. It is concluded that this method is reliable only at infection rates of 100% or higher, i.e., at one mycoplasma or more per tissue-culture cell.

📜 SIMILAR VOLUMES

3-Amino-6-methoxy-9-(2-hydroxyethylamino
3-Amino-6-methoxy-9-(2-hydroxyethylamino) acridine: A new fluorescent dye to detect mycoplasma contamination in cell cultures
✍ Chantal Jayat-Vignoles; Marie-Hélène Ratinaud; Raymond Julien 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 686 KB

A new fluorescent acridine orange derivative, 3-amino-6-methoxy-9-(2-hydroxyethylamino) acridine (AMHA), has been applied to Hela cells in order to set up appropriate conditions for the detection of mycoplasma contaminations. Since AMHA staining reveals intensely fluorescent nuclei and slight fluore