𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A new quick screening of mycoplasma contamination in cell culture by adenine detection

✍ Scribed by Masakazu Hatanaka; Richard delGuidice; Cedric Long

Publisher
Springer
Year
1976
Tongue
English
Weight
96 KB
Volume
2
Category
Article
ISSN
1573-0603

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Detection of mycoplasma contamination in
Detection of mycoplasma contamination in tissue cultures by fluorescence microscopy
✍ Maya Thangavelu; H. ErnΓΈ; A. J. Therkelsen πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 184 KB

The in situ staining method of Chen (1977) for the detection of mycoplasma contaminants in tissue cultures was tested in cultures of human skin fibroblasts after controlled contamination with Mycoplasma arginini. It is concluded that this method is reliable only at infection rates of 100% or higher,

3-Amino-6-methoxy-9-(2-hydroxyethylamino
3-Amino-6-methoxy-9-(2-hydroxyethylamino) acridine: A new fluorescent dye to detect mycoplasma contamination in cell cultures
✍ Chantal Jayat-Vignoles; Marie-HΓ©lΓ¨ne Ratinaud; Raymond Julien πŸ“‚ Article πŸ“… 1990 πŸ› Springer 🌐 English βš– 686 KB

A new fluorescent acridine orange derivative, 3-amino-6-methoxy-9-(2-hydroxyethylamino) acridine (AMHA), has been applied to Hela cells in order to set up appropriate conditions for the detection of mycoplasma contaminations. Since AMHA staining reveals intensely fluorescent nuclei and slight fluore