✦ LIBER ✦
3-Amino-6-methoxy-9-(2-hydroxyethylamino) acridine: A new fluorescent dye to detect mycoplasma contamination in cell cultures
✍ Scribed by Chantal Jayat-Vignoles; Marie-Hélène Ratinaud; Raymond Julien
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 686 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0920-9069
No coin nor oath required. For personal study only.
✦ Synopsis
A new fluorescent acridine orange derivative, 3-amino-6-methoxy-9-(2-hydroxyethylamino) acridine (AMHA), has been applied to Hela cells in order to set up appropriate conditions for the detection of mycoplasma contaminations. Since AMHA staining reveals intensely fluorescent nuclei and slight fluorescent cytoplasm, we can visualize and localize mycoplasma contamination on each cell. In combination with a shortened Chen's staining method (1977), AMHA should allow a better detection of mycoplasma in animal cell cultures than the well established Hoechst dye.