𝔖 Bobbio Scriptorium
✦   LIBER   ✦

DETECTION AND TREATMENT OF CONTAMINATING MYCOPLASMAS IN CELL CULTURE

✍ Scribed by Cross, GF ;Goodman, Margaret R ;Shaw, Elizabeth J


Book ID
109863951
Publisher
Wiley (Blackwell Publishing)
Year
1967
Weight
624 KB
Volume
45
Category
Article
ISSN
0004-945X

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The in situ staining method of Chen (1977) for the detection of mycoplasma contaminants in tissue cultures was tested in cultures of human skin fibroblasts after controlled contamination with Mycoplasma arginini. It is concluded that this method is reliable only at infection rates of 100% or higher,