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Design and kinetic characterization of a whole cell penicillin acylase biocatalyst using E. coli

✍ Scribed by Rodríguez, M.E.; Quintero, R.; López-Munguía, A.

Book ID
122991048
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
566 KB
Volume
29
Category
Article
ISSN
1873-3298

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✍ Sonia Ospina; Enrique Merino; Octavio T. Ramirez; Agustín López-Munguía 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 377 KB

The production of a whole cell biocatalyst with high specific penicillin acylase activity using a recombinant strain is described. High activities were obtained using exponentially fed-batch fermentations to overproduce the enzyme. The cells were then entrapped in agar and the activity characterized