Characterization and use of a penicillin acylase biocatalyst
✍ Scribed by Ospina, S. Sonia; Lopez-Munguia, Agustín; Gonzalez, Rosa Luz; Quintero, Rodolfo
- Book ID
- 120047567
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2007
- Tongue
- English
- Weight
- 741 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0268-2575
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📜 SIMILAR VOLUMES
The production of a whole cell biocatalyst with high specific penicillin acylase activity using a recombinant strain is described. High activities were obtained using exponentially fed-batch fermentations to overproduce the enzyme. The cells were then entrapped in agar and the activity characterized
A batch of the immobilized industrial biocatalyst glutaryl-7-ACA acylase (GA), one of the two enzymes involved in the biotransformation of cephalosporin C (CefC) into 7-aminocephalosporanic acid (7-ACA), was characterized. K(m) value for glutaryl-7-ACA was 5 mM. Enzyme activity was found to be optim