✦ LIBER ✦

Recombinant whole cell penicillin acylase biocatalyst: Production, characterization and use in the synthesis and hydrolysis of antibiotics

✍ Scribed by Sonia Ospina; Enrique Merino; Octavio T. Ramirez; Agustín López-Munguía

Publisher: Springer Netherlands
Year: 1995
Tongue: English
Weight: 377 KB
Volume: 17
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00129388

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✦ Synopsis

The production of a whole cell biocatalyst with high specific penicillin acylase activity using a recombinant strain is described. High activities were obtained using exponentially fed-batch fermentations to overproduce the enzyme. The cells were then entrapped in agar and the activity characterized in terms of particle size and stability. The technical feasibility of the catalyst in synthesis and hydrolysis reactions was demonstrated.

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