Design and construction of a syntheticBacillus thuringiensisCry4Aa gene: Hyperexpression inEscherichia coli
โ Scribed by Tohru Hayakawa; Mohammad Tofazzal Hossain Howlader; Masashi Yamagiwa; Hiroshi Sakai
- Publisher
- Springer
- Year
- 2008
- Tongue
- English
- Weight
- 142 KB
- Volume
- 80
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The Aa,A~~ tRNA gene, dnaY, and the chaperonin gene secB have been combined on the same plasmid, pHB2. Therefore, the effect of increased secB expression upon accumulation of secreted proteins may be assessed, even for genes containing a high proportion of rare arginine codons. The effect of co-tran
The glucoamylase gene from lactobacillus amylovorus was cloned and expressed in Escherichia coli. A genomic DNA library from Laclobacillus amylovorus was prepared by partially digesting genomic DNA with EC&I and ligating random fragments to the EcoRl digested cloning vector, pZErO-I .I. Three E. co/