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Gene clone and expression of a fibrinolytic enzyme (FE) inEscherichia coli

โœ Scribed by Shi-Hua Wang; Miao Diao; Yan-Ling Yang; Wei-Zhu Lin; Bi-Fang Huang

Publisher
Springer-Verlag
Year
2008
Tongue
English
Weight
239 KB
Volume
58
Category
Article
ISSN
1590-4261

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The glucoamylase gene from lactobacillus amylovorus was cloned and expressed in Escherichia coli. A genomic DNA library from Laclobacillus amylovorus was prepared by partially digesting genomic DNA with EC&I and ligating random fragments to the EcoRl digested cloning vector, pZErO-I .I. Three E. co/