✦ LIBER ✦

Cloning and expression of a glucoamylase gene fromLactobacillus amylovorusATCC 33621 inEscherichia coli

✍ Scribed by Jennylynd A. James; Normand Robert; Byong H. Lee

Publisher: Springer Netherlands
Year: 1996
Tongue: English
Weight: 457 KB
Volume: 18
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00129344

No coin nor oath required. For personal study only.

✦ Synopsis

The glucoamylase gene from lactobacillus amylovorus was cloned and expressed in Escherichia coli. A genomic DNA library from Laclobacillus amylovorus was prepared by partially digesting genomic DNA with EC&I and ligating random fragments to the EcoRl digested cloning vector, pZErO-I .I. Three E. co/i transformants expressing glucoamylase were identified using a probe prepared from the STAP glucoamylase gene from Saccharomyces cerewisiae var. diasfaticus. The physical maps of the recombinant plasmids were constructed. These plasmids contained inserts of about 5.2 Kb, 5.9 Kb and 6.4 Kb respectively. Temperature and pH optima of 45°C and 6.0, respectively, were obtained for both recombinant and purified wild type glucoamylases.

Also, the enzymes were found to be thermolabile at temperatures above 50°C.

📜 SIMILAR VOLUMES

Cloning and expression of aNostocsp. leu

Cloning and expression of aNostocsp. leucine biosynthetic gene inEscherichia coli

✍ Gerard A. Cangelosi; Cecillia M. Joseph; Joanne J. Rosen; John C. Meeks 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 771 KB

Cloning and expression of a xylanase gen

Cloning and expression of a xylanase gene fromClostridium acetobutylicumP262 inEscherichia coli

✍ Harold Zappe; David T. Jones; David R. Woods 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 635 KB

Cloning and expression inEscherichia col

Cloning and expression inEscherichia coli of the sucrase gene fromBacillus subtilis

✍ Fouet, Agnès ;Klier, André ;Rapoport, Georges 📂 Article 📅 1982 🏛 Springer 🌐 English ⚖ 816 KB

Cloning and expression of a β -1,3-gluca

Cloning and expression of a β -1,3-glucanase gene fromBacillus circulansKCTC3004 inEscherichia coli

✍ Dong Seok Lee; Hun-Gil Chang 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 459 KB

A new gene encoding the 15' -1,3-glucanase&rninarinase) of Bacillus circulans KCTC3004 was cloned into Escherichia colz using pUC19 as a vector. The gene localized in the 5.3 kb PstI DNA fragment was expressed independently of its orientation in the cloning vector showing enzyme activity about 33 ti

Molecular cloning and expression of aMic

Molecular cloning and expression of aMicromonospora chalcaeβ-glucosidase encoding gene inEscherichia coli

✍ A. Winters; J. Gallagher; N. Barron; A. Rollan; A. P. McHale 📂 Article 📅 1996 🏛 Springer Netherlands 🌐 English ⚖ 306 KB

Molecular cloning of a new endoglucanase

Molecular cloning of a new endoglucanase gene fromClostridium cellulolyticumand its expression inEscherichia coli

✍ Seigo Shima; Yasuo Igarashi; Tohru Kodama 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 481 KB

Two genes coding for endoglucanase activity in Clostridium cellulolyticum were cloned and expressed in Escherichia coli by using plasmid pUC18. The sizes of two fragments harbouring endoglucanase genes are 4.4 kb and 2.0 kb, respectively. The 2.0-kb fragment was identical with a reported D N A fragm