Molecular cloning of a new endoglucanase gene fromClostridium cellulolyticumand its expression inEscherichia coli

✍ Scribed by Seigo Shima; Yasuo Igarashi; Tohru Kodama

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 481 KB
• Volume: 35
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00184693

✦ Synopsis

Two genes coding for endoglucanase activity in Clostridium cellulolyticum were cloned and expressed in Escherichia coli by using plasmid pUC18. The sizes of two fragments harbouring endoglucanase genes are 4.4 kb and 2.0 kb, respectively. The 2.0-kb fragment was identical with a reported D N A fragment encoding an endoglucanase of C. cellulolyticum. The 4.4-kb fragment was obtained first in this study. Deletion analysis showed that a 1.3-kb portion of the 4.4-kb fragment is necessary for the endoglucanase expression by its own promoter. The 4.4-kb fragment hybridized with several different fragments of the genomic D N A in C. cellulolyticum.