Construction of a vector for probing the effect of co-expression ofdnaYandsecBupon secreted gene products inEscherichia coli

✍ Scribed by Julian A. Chesshyre; Gordon Lowe

• Publisher: Springer-Verlag
• Year: 1995
• Tongue: English
• Weight: 350 KB
• Volume: 9
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00151568

✦ Synopsis

The Aa,A~~ tRNA gene, dnaY, and the chaperonin gene secB have been combined on the same plasmid, pHB2. Therefore, the effect of increased secB expression upon accumulation of secreted proteins may be assessed, even for genes containing a high proportion of rare arginine codons. The effect of co-transformation of pHB2 upon yields of BPTI in Escherichia coli is discussed.