A new expression vector for the production of fused proteins inEscherichia coli
β Scribed by Noemi Flores; Ramon Anda; Leopoldo Guereca; Norberto Cruz; Salvador Antonio; Paulina Balbas; Francisco Bolivar; Fernando Valle
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 355 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The Aa,A~~ tRNA gene, dnaY, and the chaperonin gene secB have been combined on the same plasmid, pHB2. Therefore, the effect of increased secB expression upon accumulation of secreted proteins may be assessed, even for genes containing a high proportion of rare arginine codons. The effect of co-tran
Two fermentation processes for the tryptophan-regulated expression of active HIV protease (HIV-1 prt) in Escherichia coli are described. Since overexpression of HIV-1 prt results in cell death, stringent control of product expression was necessary to attain high enzyme levels. Such control was achie