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Density gradient fractionation by piston displacement

✍ Scribed by David H. Coombs

Book ID
102628115
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
487 KB
Volume
68
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method for the fractionation of centrifuged density gradients is described. A piston forced into the centrifuge tube from above displaces the gradient, while its tip collects liquid from the small volume immediately below it without disturbing the unfractionated part of the gradient. The centrifuge tube holder permits sensitive visual detection of bands of light scattering particles which may then be rapidly and precisely extracted. Continuous fractionation of the entire gradient is also possible.

πŸ“œ SIMILAR VOLUMES

A density gradient fractionator
A density gradient fractionator
✍ Paul T. Englund; Hamilton O. Smith; Earl Sandbek πŸ“‚ Article πŸ“… 1971 πŸ› Elsevier Science 🌐 English βš– 886 KB
Another density gradient fractionator
Another density gradient fractionator
✍ T.R. Hopkins πŸ“‚ Article πŸ“… 1973 πŸ› Elsevier Science 🌐 English βš– 153 KB

## Another Density Gradient Fractionator Density gradient centrifugation is a frequently used procedure to separate subcellular components. In its simplest form the density gradient containing the sample is removed and collected drop by drop through a hypodermic needle piercing the bottom of the p