One method of fractionating a density gradient after centrifugation is to puncture the bottom of the cent~fuge tube and then collect fractions of equal number of drops of gradient material. This method has its disadvantages. Occasionally air bubbles are introduced into the gradient
A new device for density gradient fractionation
โ Scribed by Tatsuo Oumi; Syozo Osawa
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 117 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
The excellent resolution of particles or molecules of differing molecular weight afforded by density gradient centrifugation has made this the method of choice for a variety of applications during the last decade (l-4). Concomitant with the ever-increasing interest in density gradient centrifugation
A simple, inexpensive modification of a programmable commercial fraction collector (Frac 100; Pharmacia, Sweden) was described. This modification allowed reproducible automatic fractionation of small sucrose gradients by rinsing the hanging drop at the delivery head of the fraction collector into th