An easily constructed, inexpensive, tube-puncturing device for density gradient centrifugation

✍ Scribed by Joel Oppenheim; Julian Scheinbuks; Leon Marcus

• Publisher: Elsevier Science
• Year: 1968
• Tongue: English
• Weight: 251 KB
• Volume: 25
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(68)90098-5

✦ Synopsis

The excellent resolution of particles or molecules of differing molecular weight afforded by density gradient centrifugation has made this the method of choice for a variety of applications during the last decade (l-4). Concomitant with the ever-increasing interest in density gradient centrifugation as an analytical and preparative tool has has been an increase in the number of devices for tube puncture and fraction collection (5-16). High among the obvious requirements of such devices are structural rigidity and provision for constant temperature so that the gradient will not be disturbed by tube movement or convection due to temperature change during outflow of the contents of the tube. Lesser, but still important factors, are cost and ease of construction. In the present paper we describe an inexpensive, easily constructed, tube-puncturing device * Dow Corning Lab RTV silastic silicone rubber adhesive.