Erythrocyte fractionation by velocity sedimentation and discontinuous density gradient centrifugation
โ Scribed by Arthur Houston; Gwyneth Freeman; Amy Plint; Doreen Korcock
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 855 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0920-1742
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Computer calculated isokinetic gradients provide superior uniform resolution for zone centrifugation of macromolecules and particles of known density (l-3). The technique is particularly useful in that it has the capacity to provide sedimentation coefficients without cumbersome routine computations
This work was undertaken to improve a separation method for preparation of large amounts of erythroid cells of different age with homogeneous and minimal contamination of myeloid cells. Our method was suitably employed in the study of the decay mechanism of glucose-6-phosphate dehydrogenase (G6PDH)