Improved computer program data for the resolution and fractionation of macromolecules by isokinetic sucrose density gradient sedimentation
✍ Scribed by Kenneth S. McCarty Jr.; Robin T. Vollmer; Kenneth S. McCarty
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 715 KB
- Volume
- 61
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Computer calculated isokinetic gradients provide superior uniform resolution for zone centrifugation of macromolecules and particles of known density (l-3). The technique is particularly useful in that it has the capacity to provide sedimentation coefficients without cumbersome routine computations and does not necessitate the use of internal standards (2). In addition, the technique can be utilized to predict the required centrifugation time for the resolution of two macromolecules or particles of known density. With the use of computer programs it is also possible t.o calculate sedimentation coefficients and zone sedimentation on any preselected gradient (4).
In the absence of computer facilities, however, many laboratories must depend on published computer data for specific rotors. Although some of this data has appeared in print 1967, 1968 (1,2) many new rotors are presently in use.
We wish to present here data on nine new Beckman Spinco rotors SW27.0, SW27.1, SW36, SW40-Ti, SW41-Ti, SW50.1, SW56-Ti, SW60-Ti, SW65-Ti, and new data for the SW25.1, SW25.2, SW39L and SW5OL rotors.' The computer algorithm was improved both to determine the ideal isokinet,ic sucrose concentration gradient (to .OI% accuracy) and to fit that gradient with an empirical gradient to a greater degree of accuracy than in previously published results (1-3). In addition to the data reyuired for the preparation of isokinetic sucrose density gradients the U.K values are also given to compute the sedimentation coefficient values, s2,,,s,.